Metagenomic tools in microbial ecology research

Microbial Ecology Exemplifies Building Ecology

Recent advances in molecular methods have dramatically improved knowledge of the microbial communities and their diversity in indoor environments. We have retrieved over 150 references with results of indoor microbial investigations and environmental factors. Representative papers are summarized here. The results show that buildings are complex, ecosystem-like, and that collaboration between mi...

Bioreporters in microbial ecology.

Bioreporters are effective research tools for gaining an understanding of a microbe's perception of the world. Fitted with a fusion of an environmentally responsive promoter to a suitable reporter gene, a bacterial or fungal bioreporter is able to communicate its metabolic or transcriptional behavior in a habitat, and furnish us with information on the chemical, physical or biological propertie...

PROJECT SUMMARY The Microbial Ecology of Bacterial Vaginosis: A Fine Scale Resolution Metagenomic

This manuscript is part of a pilot effort on the part of NIH staff and the Nature publishing group to provide a more convenient archive for "marker papers" to be published. These "marker papers" are designed to provide the users of community resource data sets with information regarding the status and scope of individual community resource projects. For further information see editorial in Sept...

Metagenomic pyrosequencing and microbial identification.

BACKGROUND The Human Microbiome Project has ushered in a new era for human metagenomics and high-throughput next-generation sequencing strategies. CONTENT This review describes evolving strategies in metagenomics, with a special emphasis on the core technology of DNA pyrosequencing. The challenges of microbial identification in the context of microbial populations are discussed. The developme...

Electrophoretic Techniques in Microbial Ecology